Table 1 Specific growth rate and xylose consumption rate, and fermentation profiles of wild-type BL21(DE3) strain and adapted strains with D-glucose + D-xylose, and D-xylose
From: Efficient anaerobic consumption of D-xylose by E. coli BL21(DE3) via xylR adaptive mutation
Added sugar (mM) | Strain | Fermentation time (h)a | Specific growth rate (μ) (fold) | Maximum xylose consumption rate (mM/h) (fold) | Metabolites (mM) | ||||
|---|---|---|---|---|---|---|---|---|---|
Acetate | Ethanol | Formate | Lactate | Succinate | |||||
Glucose (12.5) +Xylose (12.5) | BL21(DE3) | 42 | 0.88 ± 0.02 (1.0) | 1.1 ± 0.0 (1.0) | 22.7 ± 0.1 | 3.2 ± 0.4 | 33.6 ± 0.1 | 0.2 ± 0.0 | 12.2 ± 0.2 |
JH001 | 12 | 0.85 ± 0.03 (1.0) | 1.9 ± 0.2 (1.7) | 22.0 ± 0.9 | 15.2 ± 0.6 | 38.9 ± 1.8 | 0.2 ± 0.0 | 5.3 ± 0.1 | |
JH019 | 12 | 0.91 ± 0.07 (1.0) | 2.9 ± 0.6 (2.7) | 22.8 ± 0.8 | 14.4 ± 0.9 | 39.4 ± 1.5 | 0.2 ± 0.0 | 4.8 ± 0.3 | |
Xylose (25) | BL21(DE3) | 21 | 0.59 ± 0.04 (1.0) | 2.0 ± 0.0 (1.0) | 19.4 ± 0.3 | 7.4 ± 0.1 | 23.4 ± 0.4 | NDa | 17.9 ± 0.1 |
JH001 | 16 | 0.63 ± 0.03 (1.1) | 3.7 ± 0.6 (1.9) | 22.8 ± 0.2 | 15.1 ± 0.2 | 41.5 ± 0.4 | ND | 4.3 ± 0.1 | |
JH019 | 10 | 0.60 ± 0.02 (1.0) | 7.4 ± 0.6 (3.7) | 21.6 ± 0.1 | 13.7 ± 0.3 | 38.2 ± 0.2 | ND | 4.3 ± 0.1 | |