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Table 1 Specific growth rate and xylose consumption rate, and fermentation profiles of wild-type BL21(DE3) strain and adapted strains with D-glucose + D-xylose, and D-xylose

From: Efficient anaerobic consumption of D-xylose by E. coli BL21(DE3) via xylR adaptive mutation

Added sugar (mM) Strain Fermentation time (h)a Specific growth rate (μ) (fold) Maximum xylose consumption rate (mM/h) (fold) Metabolites (mM)
Acetate Ethanol Formate Lactate Succinate
Glucose (12.5)
+Xylose (12.5)
BL21(DE3) 42 0.88 ± 0.02 (1.0) 1.1 ± 0.0 (1.0) 22.7 ± 0.1 3.2 ± 0.4 33.6 ± 0.1 0.2 ± 0.0 12.2 ± 0.2
JH001 12 0.85 ± 0.03 (1.0) 1.9 ± 0.2 (1.7) 22.0 ± 0.9 15.2 ± 0.6 38.9 ± 1.8 0.2 ± 0.0 5.3 ± 0.1
JH019 12 0.91 ± 0.07 (1.0) 2.9 ± 0.6 (2.7) 22.8 ± 0.8 14.4 ± 0.9 39.4 ± 1.5 0.2 ± 0.0 4.8 ± 0.3
Xylose (25) BL21(DE3) 21 0.59 ± 0.04 (1.0) 2.0 ± 0.0 (1.0) 19.4 ± 0.3 7.4 ± 0.1 23.4 ± 0.4 NDa 17.9 ± 0.1
JH001 16 0.63 ± 0.03 (1.1) 3.7 ± 0.6 (1.9) 22.8 ± 0.2 15.1 ± 0.2 41.5 ± 0.4 ND 4.3 ± 0.1
JH019 10 0.60 ± 0.02 (1.0) 7.4 ± 0.6 (3.7) 21.6 ± 0.1 13.7 ± 0.3 38.2 ± 0.2 ND 4.3 ± 0.1
  1. aFermentation time (h) when glucose plus xylose or xylose were completely consumed
  2. aND Not detected