Table 5 Cycling conditions for re-amplification of ddPCR
From: ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples
Cycling step | Temperature (°C) | Time | Number of cycles |
|---|---|---|---|
Initial denaturation | 98 | 30 s | 1 |
Denaturation | 95 | 10 s | 5 |
Annealing | 55 | 20 s | |
Extension | 72 | 45 s | |
Final extension | 90 | 5 min | 1 |
Hold | 4 | ∞ | 1 |