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Table 5 Cycling conditions for re-amplification of ddPCR

From: ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples

Cycling step Temperature (°C) Time Number of cycles
Initial denaturation 98 30 s 1
Denaturation 95 10 s 5
Annealing 55 20 s
Extension 72 45 s
Final extension 90 5 min 1
Hold 4 1