Skip to main content

Table 3 Variable region-specific forward and reverse primers and annealing temperature for 1st-step PCR

From: ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples

Region Forward primer Reverse primer Annealing temperature Reference
V1-V2 AGA GTT TGA TYM TGG CTC AG GCT GCC TCC CGT AGG AGT 57 °C Salter, et al. [28]
V3-V4 CCT ACG GGN GGC WGC AG GAC TAC HVG GGT ATC TAA TCC 55 °C Klindworth, et al. [10]
V7-V9 CAA CGA GCG CAA CCC T GGT TAC CTT GTT ACG ACT T 51 °C Turner, et al. [62]