Table 3 Variable region-specific forward and reverse primers and annealing temperature for 1st-step PCR
From: ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples
Region | Forward primer | Reverse primer | Annealing temperature | Reference |
|---|---|---|---|---|
V1-V2 | AGA GTT TGA TYM TGG CTC AG | GCT GCC TCC CGT AGG AGT | 57 °C | Salter, et al. [28] |
V3-V4 | CCT ACG GGN GGC WGC AG | GAC TAC HVG GGT ATC TAA TCC | 55 °C | Klindworth, et al. [10] |
V7-V9 | CAA CGA GCG CAA CCC T | GGT TAC CTT GTT ACG ACT T | 51 °C | Turner, et al. [62] |