Table 2 Concentrations and dilutions of DNA input used for 1st-step PCR reaction
From: ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples
Name | Total input DNA (ng) | Final DNA concentration in 50 μl PCR reaction (pg/μl) |
|---|---|---|
60 | 60 | 1200 |
12 | 12 | 240 |
10 | 10 | 200 |
5 | 5 | 100 |
1 | 1 | 20 |
0.5 | 0.5 | 10 |
0.1 | 0.1 | 2 |
0.05 | 0.05 | 1 |
0.01 | 0.01 | 0.2 |