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Table 2 Concentrations and dilutions of DNA input used for 1st-step PCR reaction

From: ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples

Name Total input DNA (ng) Final DNA concentration in 50 μl PCR reaction (pg/μl)
60 60 1200
12 12 240
10 10 200
5 5 100
1 1 20
0.5 0.5 10
0.1 0.1 2
0.05 0.05 1
0.01 0.01 0.2