Fig. 4
From: ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples
Proof of concept study for low biomass samples using water of ponds and rivers. A Ten different samples were selected from water bodies in and around the city of Freising, Germany. The map was provided by GoogleMaps. B The rarefaction curve shows that cleaned water samples (MilliQ and desalted water samples) have significantly lower number of reads and species compared to all other water body samples. C The multi-dimensional scaling (MDS) plot shows that cleaned water samples (‘control’) cluster significantly apart from the water body samples. D Significant differences were found when comparing the water body samples to the controls for richness or, e.g., the abundance of the genus Limnohabitans