Fig. 2
From: ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples
Control experiment to test for bias possibly introduced due to the extra ddPCR step after 2nd-step PCR. Samples processed with ddPCR (Samples marked “D” for ddPCR processed, 12 ng DNA used) are compared to standard short amplicon controls which were not ddPCR processed (Samples marked “C” for Control, 12 ng DNA used). Four human samples: T1 (red), T28 (orange), T29 (green), T30 (turquoise) and two mock communities: Zymo (pink), ZIEL2 (blue) were sequenced using primer pairs amplifying different V-regions. A Meta Multi-Dimensional Scaling (MDS) shows that samples cluster significantly differently due to their origin and not by preparation method. B The dendrogram shows that clustering is dependent on sample origin even though clustering within a sample is effected by the V-region targeted. C Taxonomic profiles at genus-level of Sample-C and Sample-D for human samples. D As before, for mock samples from Zymo and (E) and ZIEL2. Note, the taxonomic profiles at the genus-level show only minor differences when the same V-region is targeted