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Table 3 Mutations in associated colistin-resistance proteins in E. coli, K. pneumoniae and A. bereziniae resistant isolates

From: Colistin resistance in Gram-negative bacteria analysed by five phenotypic assays and inference of the underlying genomic mechanisms

Species Isolate MIC (mg/L)a Amino acid change Plasmid mediated resistance
MgrB PmrB PmrA PmrC PhoP PhoQ mcr
K. pneumoniae 404,507-16 ≥64 D31N L213M A41T    S288N  
4,002,006-2 32 K3*       
16,003,084 32 C28S   A217V G25S    
20,038,016 32 C39Y       
808,927-16 32 L8*    R152H    
D477N
26,048,671 32 I41* (ISKpn26)b       
800,138-16 32 I41* (ISEcp1)b       
401,433-14 16 Q30*       
802,208-17 16 L4*   D149E     
187,701,876 16 C39G       
19,852,760 8    A217V G25S    
809,156-16 4    D149E     
E. coli 721,296-16 16   P97L   C27Y    
700,455-17 16   L197D   N12D    
709,006-16 16   E169K      
705,963-16 16   P97A      
700,099-17 8        mcr 1.1
706,090-16 8    R81H Q479E    
NCTC-13846 8       L467M mcr 1.1
KP-37-MCR-2-18 8     C27Y    mcr 2
L74I
707,671-17 8     C27Y    
719,645-16 8        mcr 1.1
705,498-12 4        mcr 1.1
A. bereziniae 502,814-14 ≥64   Q242R      
  1. aMIC values obtained by the reference broth microdilution method
  2. bNucleotide sequence interrupted by insertion sequence. * indicates premature stop codons or termination in the amino acid sequence indicates premature stop codons or termination in the amino acid sequence