Table 3 Primers and PCR protocols
Target DNA | Primer | Primer sequence 5′–3’ | PCR protocol |
|---|---|---|---|
LSU rDNA | LR0R LR5 | ACCCGCTGAACTTAAGC TCCTGAGGGAAACTTCG | 95 °C for 3 min; 31 cycles of 94 °C for 30 s, 50 °C for 45 s, and 72 °C for 1 min 30 s; 72 °C for 7 min; and 4 °C on hold |
ITS rDNA | ITS5 ITS4 | GGAAGTAAAAGTCGTAACAAGG TCCTCCGCTTATTGATATGC | 95 °C for 3 min; 31 cycles of 94 °C for 1 min, 50 °C for 45 s, and 72 °C for 3 min; 72 °C for 7 min; and 4 °C on hold |