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Table 3 Phylogenetic relationships between S. aureus clonal complexes, using the concatenated gene sequences from the MLST database

From: Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon

Microrganism Gene Nucleotide composition (%) Lenght (n) Variable sites (S) S/n (Ps) Θ π Tajima’s D
A C G T
S. aureus arcC 36.4 17.1 21.4 25.2 456 67 0.1469 0,0186 0.0068 −1.6654
aroE 36.0 11.3 18.9 33.8 456 70 0.1535 0.0205 0.0099 −1.3612
glpF 25.7 16.3 24.5 33.5 465 112 0.24 0.0318 0.0044 −2.3522
gmK 37.2 12.4 21.2 29.3 417 53 0.127 0.0164 0.0083 −1.2761
ptA 35.8 16.6 19.5 28.0 474 196 0.4135 0.0618 0.0068 −2.4876
tpI 36.6 15.1 22.6 25.8 402 139 0.3458 0.0487 0.0096 −2.2167
yqiL 34.0 15.6 22.4 28.0 516 68 0.1318 0.0162 0.0065 −1.5817
S. epidermidis arcC 36.8 19.2 17.0 27.1 468 72 0.1538 0,0190 0.0153 −0.5227
aroE 37.1 11.8 17.8 33.3 420 78 0.1857 0.0230 0.0110 −1.3911
gtr 28.7 20.4 14.9 36.0 438 86 0.1963 0.0244 0.0119 −1.3708
mutS 30.4 19.8 11.4 38.4 412 48 0.1165 0.0144 0.0054 −1.6011
pyrR 34.4 16.1 21.9 27.5 424 57 0.1344 0.0167 0.0086 −1.2572
tpiA 34.6 16.0 22.0 27.4 424 67 0.1580 0.0196 0.0036 −2.1526
yqiL 31.6 15.2 21.5 31.6 416 62 0.1490 0.0185 0.0079 −1.4948
  1. Nucleotide frequencies and parameters associated with the Tajima neutrality test for each MLST gene analysed. A, C, G and T are the percentages for each nucleotide in the genes in question. Size (n) corresponds to the total number of sites in each gene. Variable sites (S) correspond to the sites that showed nucleotide changes during alignments. Ps corresponds to the ratio S/n. Θ and π correspond to values that measure nucleotide diversity. Tajima’s D corresponds to the reference parameter to verify the neutral theory of evolution for each gene. All values of Tajima’s D showed statistical significance P < 0.01