|
Microrganism
|
Gene
|
Nucleotide composition (%)
|
Lenght (n)
|
Variable sites (S)
|
S/n (Ps)
|
Θ
|
π
|
Tajima’s D
|
|---|
|
A
|
C
|
G
|
T
|
|---|
|
S. aureus
|
arcC
|
36.4
|
17.1
|
21.4
|
25.2
|
456
|
67
|
0.1469
|
0,0186
|
0.0068
|
−1.6654
|
|
aroE
|
36.0
|
11.3
|
18.9
|
33.8
|
456
|
70
|
0.1535
|
0.0205
|
0.0099
|
−1.3612
|
|
glpF
|
25.7
|
16.3
|
24.5
|
33.5
|
465
|
112
|
0.24
|
0.0318
|
0.0044
|
−2.3522
|
|
gmK
|
37.2
|
12.4
|
21.2
|
29.3
|
417
|
53
|
0.127
|
0.0164
|
0.0083
|
−1.2761
|
|
ptA
|
35.8
|
16.6
|
19.5
|
28.0
|
474
|
196
|
0.4135
|
0.0618
|
0.0068
|
−2.4876
|
|
tpI
|
36.6
|
15.1
|
22.6
|
25.8
|
402
|
139
|
0.3458
|
0.0487
|
0.0096
|
−2.2167
|
|
yqiL
|
34.0
|
15.6
|
22.4
|
28.0
|
516
|
68
|
0.1318
|
0.0162
|
0.0065
|
−1.5817
|
|
S. epidermidis
|
arcC
|
36.8
|
19.2
|
17.0
|
27.1
|
468
|
72
|
0.1538
|
0,0190
|
0.0153
|
−0.5227
|
|
aroE
|
37.1
|
11.8
|
17.8
|
33.3
|
420
|
78
|
0.1857
|
0.0230
|
0.0110
|
−1.3911
|
|
gtr
|
28.7
|
20.4
|
14.9
|
36.0
|
438
|
86
|
0.1963
|
0.0244
|
0.0119
|
−1.3708
|
|
mutS
|
30.4
|
19.8
|
11.4
|
38.4
|
412
|
48
|
0.1165
|
0.0144
|
0.0054
|
−1.6011
|
|
pyrR
|
34.4
|
16.1
|
21.9
|
27.5
|
424
|
57
|
0.1344
|
0.0167
|
0.0086
|
−1.2572
|
|
tpiA
|
34.6
|
16.0
|
22.0
|
27.4
|
424
|
67
|
0.1580
|
0.0196
|
0.0036
|
−2.1526
|
|
yqiL
|
31.6
|
15.2
|
21.5
|
31.6
|
416
|
62
|
0.1490
|
0.0185
|
0.0079
|
−1.4948
|
- Nucleotide frequencies and parameters associated with the Tajima neutrality test for each MLST gene analysed. A, C, G and T are the percentages for each nucleotide in the genes in question. Size (n) corresponds to the total number of sites in each gene. Variable sites (S) correspond to the sites that showed nucleotide changes during alignments. Ps corresponds to the ratio S/n. Θ and π correspond to values that measure nucleotide diversity. Tajima’s D corresponds to the reference parameter to verify the neutral theory of evolution for each gene. All values of Tajima’s D showed statistical significance P < 0.01
