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Table 3 Phylogenetic relationships between S. aureus clonal complexes, using the concatenated gene sequences from the MLST database

From: Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon

Microrganism

Gene

Nucleotide composition (%)

Lenght (n)

Variable sites (S)

S/n (Ps)

Θ

π

Tajima’s D

A

C

G

T

S. aureus

arcC

36.4

17.1

21.4

25.2

456

67

0.1469

0,0186

0.0068

−1.6654

aroE

36.0

11.3

18.9

33.8

456

70

0.1535

0.0205

0.0099

−1.3612

glpF

25.7

16.3

24.5

33.5

465

112

0.24

0.0318

0.0044

−2.3522

gmK

37.2

12.4

21.2

29.3

417

53

0.127

0.0164

0.0083

−1.2761

ptA

35.8

16.6

19.5

28.0

474

196

0.4135

0.0618

0.0068

−2.4876

tpI

36.6

15.1

22.6

25.8

402

139

0.3458

0.0487

0.0096

−2.2167

yqiL

34.0

15.6

22.4

28.0

516

68

0.1318

0.0162

0.0065

−1.5817

S. epidermidis

arcC

36.8

19.2

17.0

27.1

468

72

0.1538

0,0190

0.0153

−0.5227

aroE

37.1

11.8

17.8

33.3

420

78

0.1857

0.0230

0.0110

−1.3911

gtr

28.7

20.4

14.9

36.0

438

86

0.1963

0.0244

0.0119

−1.3708

mutS

30.4

19.8

11.4

38.4

412

48

0.1165

0.0144

0.0054

−1.6011

pyrR

34.4

16.1

21.9

27.5

424

57

0.1344

0.0167

0.0086

−1.2572

tpiA

34.6

16.0

22.0

27.4

424

67

0.1580

0.0196

0.0036

−2.1526

yqiL

31.6

15.2

21.5

31.6

416

62

0.1490

0.0185

0.0079

−1.4948

  1. Nucleotide frequencies and parameters associated with the Tajima neutrality test for each MLST gene analysed. A, C, G and T are the percentages for each nucleotide in the genes in question. Size (n) corresponds to the total number of sites in each gene. Variable sites (S) correspond to the sites that showed nucleotide changes during alignments. Ps corresponds to the ratio S/n. Θ and π correspond to values that measure nucleotide diversity. Tajima’s D corresponds to the reference parameter to verify the neutral theory of evolution for each gene. All values of Tajima’s D showed statistical significance P < 0.01