Multiple Cross Displacement Amplification Coupled with Lateral Flow Biosensor (MCDA-LFB) for rapid detection of Legionella pneumophila

Table 1 The Primers Used in the Current Report

Primers Name^a	Sequences and modifications^b	Length^c	Gene
F1	5′-GAAATGGTGTTAAACCCGG-3′	19 nt	mip
F2	5′-AAGTTTCATTTGGGCCAAT-3′	19 nt
CP1	5′-GGTACTGTCAAAAACGGTACCACGGATACAGTCACTGTCGA-3′	41mer
CP2	5′-TGGATGGACAGAAGCTTTGCGGCCATATGCAAGACCTGA-3′	39mer
C1*	5′-Biotin-GGTACTGTCAAAAACGGTACCA-3’	22mer
C2	5′-TGGATGGACAGAAGCTTTGC-3’	20 nt
D1*	5′-FITC-TCAATCAGACGACCAGT-3’	17 nt
D2	5′-TGCCAGCTGGATCAACTT-3’	18 nt
R1	5′-GTTGCTGGCTTACCAGT-3’	17 nt
R2	5′-GTTCCAGGTTTCACAAGTT-3’	19 nt

^aC1∗, 5′-labeled with biotin when used in MCDA-LFB assay; D1∗, 5′-labeled with FITC when used in MCDA-LFB assay
^bFITC, fluorescein isothiocyanate
^cnt, nucleotide; mer, monomeric

ISSN: 1471-2180