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Table 1 The Primers Used in the Current Report

From: Multiple Cross Displacement Amplification Coupled with Lateral Flow Biosensor (MCDA-LFB) for rapid detection of Legionella pneumophila

Primers Namea Sequences and modificationsb Lengthc Gene
F1 5′-GAAATGGTGTTAAACCCGG-3′ 19 nt mip
F2 5′-AAGTTTCATTTGGGCCAAT-3′ 19 nt
CP1 5′-GGTACTGTCAAAAACGGTACCACGGATACAGTCACTGTCGA-3′ 41mer
CP2 5′-TGGATGGACAGAAGCTTTGCGGCCATATGCAAGACCTGA-3′ 39mer
C1* 5′-Biotin-GGTACTGTCAAAAACGGTACCA-3’ 22mer
C2 5′-TGGATGGACAGAAGCTTTGC-3’ 20 nt
D1* 5′-FITC-TCAATCAGACGACCAGT-3’ 17 nt
D2 5′-TGCCAGCTGGATCAACTT-3’ 18 nt
R1 5′-GTTGCTGGCTTACCAGT-3’ 17 nt
R2 5′-GTTCCAGGTTTCACAAGTT-3’ 19 nt
  1. aC1, 5′-labeled with biotin when used in MCDA-LFB assay; D1, 5′-labeled with FITC when used in MCDA-LFB assay
  2. bFITC, fluorescein isothiocyanate
  3. cnt, nucleotide; mer, monomeric