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Table 1 Primer specifications designed to amplify the VioA and EstA/B genes of Janthinobacterium sp.

From: Molecular signatures of Janthinobacterium lividum from Trinidad support high potential for crude oil metabolism

Primer name

Primer orientation

5′ to 3′ Primer sequence

Start nucleotide position

Primer length

Tma

% GC

PCR product/bp

VioA gene-Janthino

Forward Primer

TCGAGTTCGTCAGCCATTAC

365

20

61.7

50

 

Reverse Primer

CTTCTTCTTCCGTCCGTTGA

1264

20

61.7

50

900

EstA/B-Janthino

Forward Primer

GTTGATGCTGCTGCAAGTG

24

19

61.9

52.6

 

Reverse Primer

TGTCGTGATGCGAATAGATCG

784

21

62.1

47.6

761

  1. aTm PCR annealing temperature