Fig. 4From: Detection of viable and total fungal community in zaopei of Chinese strong-flavor baijiu using PMA combined with qPCR and HTS based on ITS2 regionThe effectiveness of PMA to remove the dead bacteria DNA in zaopei with adding heated E. coli cells. The zaopei samples (n = 4) were collected on the 7th, 15th, 30th and 60th day of fermentation (7 d, 15 d, 30 d, and 60 d) from 20-year pit. E. coli PMA (−): the positive control (dead E. coli cells and 1 mL zaopei suspension) was untreated with PMA; E. coli PMA (+): the positive control was treated with PMA. The Rank sum test was used to calculate the significant difference by R software (* represents significant difference)Back to article page