Table 2 Bacterial strains and plasmids used in this study
Strains/plasmids | Genotype or relevant characteristicsa | Source or reference |
|---|---|---|
Plesiomonas shigelloides strains | ||
 G5884 | Wild type, serotype O45:H2b | CNCTCb Aer 44/89 |
 △arcA | arcA gene deletion mutant of G5884 | This study |
 ΔarcA/pBAD33-arcA+ | ΔarcA containing pBAD33 carrying arcA ORF with its own promoter | This study |
E. coli strains | ||
 DH5α λpir | Transformation host | Lab collection |
 S17–1 λpir | TpR SmRrecA, thi, pro, hsdR-M + RP4: 2-Tc:Mu: Km Tn7 λpir, Kmr, Smr, Tpr | [62] |
 BL21(DE3) | Host strain for protein expression | Lab collection |
 BL21/ pET28a-arcA+ | BL21(DE3) with pET28a carrying the arcA ORF; Kmr | This study |
Plasmids | ||
 pRE112 | Widely used gene knocked vector, with onT RP4, Cmr | [63] |
 pBAD33 | Arabinose inducible expression vector, CmR | [64] |
 pET28a | T7 expression vector; Kmr | Lab collection |
 pRE112-arcA− | pRE112 containing the homologous arms of arcA gene of G5884, Cmr | This study |
 pBAD33-arcA+ | pBAD33 with complete arcA | This study |
 pET28a-arcA+ | pET28a carrying the arcA gene; Kmr | This study |