Fig. 2

Confirmation of the deletion and complementation of arcA in P. shigelloides. A Graphical process of the deletion of the arcA gene. B PCR detection of the product. 1, DL2000 DNA marker; 2, PCR fragment of SX; 3, PCR amplicon of S-arcA-X from the WT genomic DNA; 4, PCR amplificon of SX from the ΔarcA genome DNA; 5, PCR amplification of arcA from the ΔarcA genome DNA; 6, PCR amplification of arcA from the WT genome DNA. C 1, DL2000 DNA marker; 2, PCR amplification of pBAD33-UD from the pBAD33 plasmid; 3, PCR amplification of pBAD33-U-arcA-D from the arcA⁺ complementation strain; 4, PCR amplification of arcA from the genomic DNA of the complementation strain