Fig. 4

Longitudinal analysis of donor strains and recipient strains in recipients post-FMT. The WSS scores were determined by comparing the donor’s sample to every available paired recipient’s sample. In addition, the recipient’s pre-FMT sample was also compared with the same recipient’s post-FMT samples to determine strain relatedness. Only donor-recipient pairs that responded to anti-PD-1 IT were included for this analysis. (A) a donor of 18 − 0002 was used to multiple recipients (19–0023, 18–0018) for FMT; and (B) donor of 18 − 0005 was used to multiple recipients (19 − 0001, 18 − 0007). Only two donor-recipient (18 − 0002 vs. 18–0018 and 18 − 0005 vs. 18 − 0007) of four pairs were responded to the anti-PD-1 IT, and the WSS scores for all pairs used in this analysis are provided in Table S3. All samples used for this analysis are listed in Table S1. The WSS scores per each donor-recipient pair were grouped into different color boxes (see the figure key). Major species found in the fecal sample and common ones found in the two different data sets (Baruch et al. and Davar et al.) are included in this figure. The remaining species found in this data set are displayed in Fig. S4. The white boxes indicate the microbial strains that we were unable to reliably determine relatedness due to the any sample in pairs not satisfying the criteria of WSS analysis (minimum coverage > 30 % and average depth > 3.5)