Detection of Legionella species, the influence of precipitation on the amount of Legionella DNA, and bacterial microbiome in aerosols from outdoor sites near asphalt roads in Toyama Prefecture, Japan

Table 3 Analysis of air samples containing Legionella-assigned reads at the species level

Sample No.	Sampling source type	qPCR (copies/m³)	qPCR with amoebic co-culture (copies/m³)	No. of reads assigned to:				Total	Number of clean sequences	Detection rate of Legionella species (%)
Sample No.	Sampling source type	qPCR (copies/m³)	qPCR with amoebic co-culture (copies/m³)	L. birminghamensis	L. geestiana	L. nautarum	L. pneumophila	Total	Number of clean sequences	Detection rate of Legionella species (%)
22	Road	161.6	0.0	0	0	0	31	31	33,964	0.09
35	Road	245.7	15.8	0	33	0	0	33	185,688	0.02
86	Road	6.3	0.0	0	0	0	122	122	114,647	0.11
98	Bathroom	65.7	0.0	0	0	0	165	165	112,066	0.15
107	Road	365.0	67.7	1743	0	0	0	1743	215,097	0.81
159	Indoor site	72.1	Not tested	0	0	400	0	400	50,889	0.79

ISSN: 1471-2180