Fig. 4
Expression of VacA recombinant protein in the supernatant and pellet as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting. A and B Expression of VacA recombinant protein in the supernatant as detected by SDS-PAGE (left) and western blotting (right); C and D Expression of VacA recombinant protein in the pellet as detected by SDS-PAGE (left) and western blotting (right). BSA: bovine serum albumin(2.00 μg); R: VacA34–854 (2.00 μg); Both M1 and M2 are protein markers