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Fig. 1 | BMC Microbiology

Fig. 1

From: Construction and preservation of a stable and highly expressed recombinant Helicobacter pylori vacuolating cytotoxin A with apoptotic activity

Fig. 1

Successful construction of the recombinant plasmid. A. The construction diagram of a vacA carrier: pET-41b-vacA34–854. Gene name: vacA34–854; Gene length: 2502 bp; Carrier name: pET-41b; Enzyme cutting sites: NdeI + XhoI; C terminal integrates the 8*His label. B. pET-41b-vacA34–854 enzyme cutting identification. M: 1 Kb DNA ladder; Lane 1: recombinant plasmid pET-41b-vacA34–854 cut by NdeI & XhoI endonucleases. C. Positive clone sequencing of pET41b-VacA34–854 recombinant plasmid

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BMC Microbiology

ISSN: 1471-2180

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