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Table 1 Kinetic constants for variable acyl-ACP substrates reacting with AhyI

From: Structural insights into acyl-ACP selective recognition by the Aeromonas hydrophila AHL synthase AhyI

Variable acyl-ACPs

kcat (s−1) × 10–3

Km (M) ×  10–6

kcat/Km (M− 1s − 1) ×  10–3

Relative ratioa

C4-ACP

22.73 ± 3.2

1.85 ± 0.16

12.29 ± 1.61

1

C6-ACP

19.44 ± 2.4

13.18 ± 1.9

1.48 ± 0.13

0.12

C8-ACP

8.41 ± 0.79

41.37 ± 8.9

0.20 ± 0.05

0.016

C10-ACP

6.34 ± 0.43

91.79 ± 15.5

0.07 ± 0.01

0.0057

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BMC Microbiology

ISSN: 1471-2180

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