A rapid and efficient method for the extraction and identification of menaquinones from Actinomycetes in wet biomass

Table 1 Concentration of menaquinones (MKs) extracted by the LCM method and the Collins method

Strains	Menaquinone concentration (mg/g DCW)		Fold change
Strains	LCM method (n = 3)	Collins method (n = 3)	Fold change
Brachybacterium squillarum JCM 16464^T	0.664 ± 0.040	0.020 ± 0.003	33.2
Brevibacterium linens JCM 1327^T	0.110 ± 0.011	0.027 ± 0.002	4.1
Chryseoglobus frigidaquae JCM 14730^T	0.151 ± 0.009	0.041 ± 0.007	3.7
Georgenia subflava Y32^T	0.921 ± 0.084	0.054 ± 0.007	17.1
Janibacter melonis JCM 16063^T	0.715 ± 0.079	0.591 ± 0.041	1.2
Microbacterium yannicii JCM 18959^T	0.201 ± 0.014	0.041 ± 0.005	4.9
Microbacterium ginsengiterrae JCM 15516^T	0.322 ± 0.035	0.021 ± 0.002	15.3
Microbacterium ureisolvens CFH S00084^T	0.355 ± 0.022	0.001 ± 0.001	355.0
Microbacterium hibisci CCTCC AB 2016180^T	0.081 ± 0.031	0.046 ± 0.003	1.8
Nesterenkonia halobia JCM 15475^T	0.100 ± 0.010	0.009 ± 0.001	11.1
Saccharopolyspora coralli E2A^T	0.063 ± 0.006	0.012 ± 0.001	5.3
Yonghaparkia alkaliphile JCM 15138^T	0.087 ± 0.001	0.022 ± 0.002	4.0
Streptomyces indicus MCCC 1A03308^T	0.214 ± 0.007	0.163 ± 0.006	1.3

ISSN: 1471-2180