Table 3 PCR primers used in this study
Description | Primers | Sequence (5' to 3') | Tm (ËšC) | % GC | amplicon size (bp) |
|---|---|---|---|---|---|
Primers used to detect McProf phage attachment sites in M. chelonae | Mc_attL_F | CGTCACGTTGGGGACTATCT | 56.5 | 55 | 212 |
Mc_attL_R | TTGAGCTGCGGATAACCTCT | 56 | 50 | ||
Mc_attR_F | CGCTTGTAATCGTCGTCGTA | 54.7 | 50 | 1067 | |
McProphageRR | ATAACTTTCGGCGGTTCCTT | 54.5 | 45 | ||
Primers used to detect BPs phage attachment sites in M. chelonae | BPs_attP_L | GCTTTATCCAGGGTTGACCA | 54.8 | 50 | 203 |
BPs_attP_R | GTTCCGATTAGTTGGCTGGA | 54.8 | 50 | ||
BPs_attB_L | GTCTCGTTACTGGCGAGCTT | 57.1 | 55 | 548 | |
BPs_attB_R | CGGGTAGTAGGCAGATGAGC | 57.2 | 60 |