Fig. 5

HSPG and β1 integrin cooperate to induce PagN-mediated internalization. a-b Parental F9, triple knockout (TKO) and double knockout (DKO) cells were infected with HB101-pagN at MOI 1:10 at 37 °C for 1 h. a Percentages of total cell-associated bacteria and (b) internalized bacteria were determined as described in the Materials and Methods. Results shown are expressed relative to values got with the parental F9 cells (F9), set at 100%. c-d pgsA745 cells were untreated or treated with integrin β1 blocking antibody and IgG at 50 μg/mL for 30 min at 4 °C prior to the addition of HB101-pagN or HB101-psup at MOI 1:10 for 1 h at 37 °C. c Percentages of total cell-associated bacteria and (d) internalized bacteria were determined as described in the Materials and Methods. Results obtained are expressed relative to values obtained for untreated cells infected with HB101-pagN, arbitrarily set at 100%. Results represent means ± SD of three independent experiments with two infected wells per experiment. Results were compared using a Mann Whitney test (****p < 0.0001, **p < 0.01)