Fig. 1
Characterization of K. phaffii puromycin sensitivity. For the growth curves, OD600 was measured every 15 min in a microplate reader for 24 h at 30 °C and 550 rpm. All growth curves were measured in biological triplicates. The lines represent average values. As controls, untreated cells were included on each assay plate. a-d K. phaffii CBS2612 (a and b) and the erg6Δ mutant (CBS7435 in c and CBS2612 in d) were cultivated in complex media YPD (YP + 2% glucose; a and c), and minimal media (ASMv6 + 2% glucose; b and d), respectively, with different concentrations of puromycin. In a-d, green symbols depict the control without puromycin in complex media, blue symbols are the control in minimal media. e μ derived from growth curves of K. phaffii CBS2612 cultivations, showing puromycin susceptibility when treated with different surfactants. Blue bars show the results obtained in complex media, while green bars show the results in minimal media