Fig. 6

TnSeq - Structure of transposon insertions and reads in Santiago et al. [2] technique. a Structure of a transposon construct insertion. The transposon can insert in two directions since a TA site is identical in both strands. The MmeI insertion in the transposon allows reads of uniform size by cutting DNA 20 bases upstream of the transposon. b Structure of the reads. In addition to the fragment of 20bp upstream of the MmeI site, the reads contain an Illumina adapter P7 containing a 3bp barcode specific to the type of construct and an Illumina adapter P5 containing a sample barcode at the 5’ end. The read also contains the MmeI transcription site included in the ITR region. We can see from that structure that the insertion site’s position is aligned with the 3’ end of the read