Fig. 2

Colony count. a. Image data processing. The left image is a representative CCD camera photograph of the monogenetic E. coli colonies formed on the agar plate as an experimental result. The right image shows the colony count and evaluation with the newly developed computational program. b. Comparison of automatic and manual counts of colonies. The numbers of colonies formed on the agar plate were counted by eye (manual) and by the original Java script add-in program executed in Fiji software (automatic) and were plotted against each other. The linear regression is indicated by the red solid line, and its significance (R²) is shown