Fig. 6

Changes in the intrahepatic inflammatory response with progression of liver disease demonstrating a shift from a proinflammatory to an immunosuppressed profile as HCC ensues. Fold change from baseline of key intrahepatic genes with progressive liver injury/disease in Mdr2 −/− mice (liver inflammation/Mdr2−/−, n = 4; liver cirrhosis/Mdr2−/−, n = 4 and advanced cirrhosis/hepatocellular carcinoma (HCC)/Mdr2−/−, n = 4). Advanced cirrhosis represents the peritumoral tissue at 42 weeks, whilst HCC represents the tumor tissue proper at 42 weeks. The mean gene expression level of the baseline/WT timepoint (n = 4) is represented as the dashed horizontal line, from which log₂ fold change (Log₂FC) is calculated; a value above the dashed line represents an increased gene expression compared to baseline/WT, and a value below the dashed line represents decreased gene expression compared to baseline/WT; box plots indicate median (middle line), 25th, 75th percentile (box) and 10th and 90th percentile (whiskers) as well as outliers (single points). P values are calculated by one-way ANOVA for overall comparison and Tukey’s test for 2 group comparison. *P < 0.05; ** P < 0.01; *** P < 0.001 and **** P < 0.0001. Detailed data is shown in Supporting Fig. 6, Additional File 1 and Supporting Table 4, Additional File 2