Fig. 4

Western Blot Overlay. Collagen type I, collagen type IV, laminin, plasminogen, plasma fibronectin, fibrinogen, and BSA (negative control) were subjected to 4–20% gradient gel (SDS PAGE) under reducing conditions, transferred to nitrocellulose membranes and stained with Ponceau S (right). After incubation of membrane with recombinant proteins, the binding was detected with polyclonal antibodies recognizing Lp25, LigBC and GroEL. Chains of host proteins are indicated by γ (400 kDa), β (270 kDa), α2 (130 kDa), α1 (115 kDa) of collagen type I; α1 (140 kDa), α2 (160 kDa) of collagen type IV; C1 (400 kDa) and C2 (200 kDa) of laminin; α (220 kDa) and β (212 kDa) of plasma fibronectin; α (63.5 kDa), β (56 kDa), γ (47 kDa) of fibrinogen; plasminogen and BSA with 70 kDa and 66 kDa, respectively. Lp25 and LigBC were used as negative and positive binding controls, respectively. Full-length Western blot overlays are shown in the Supplementary Material as Fig. S5