Fig. 2

E. coli DualX-B15(DE3) was generated by incorporation of a premature stop codon into the rnc14 gene of the donor strain BL21(DE3). Alignments of the amino acid and nucleotide sequences of the rnc14 gene from the BL21(DE3) (Top) and DualX-B15(DE3) (Bottom) strains from the start codon (ATG). The asterisk at the end of the sequence of DualX-B15(DE3) indicates the premature stop codon (TAA). The nucleotide sequence inserted into the BL21(DE3) genome during P1 phage transduction is shown as blue underlined letters. The chromatogram from Sanger sequencing in the panel below showed the insertion mutation and the premature stop codon in the rnc14 gene