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Table 3 Oligonucleotides used in the current study

From: Utilization of L-glutamate as a preferred or sole nutrient in Pseudomonas aeruginosa PAO1 depends on genes encoding for the enhancer-binding protein AauR, the sigma factor RpoN and the transporter complex AatJQMP

Oligonucleotides

Sequence

Purposea

BL342.r

gcagttatttttgacaccagaccaactggta

E. coli lacZ

JS05.r

tgaatccgtaatcatggtcatgttggggtttcccctcgg

aatQ-lacZ

JS06.f

ccgaggggaaaccccaacatgaccatgattacggattcactgg

E. coli lacZ (aatQ overlap)

JS42.f

ccgaggggaaaccccaacatgaccatgattacggattcactgg

E. coli lacZ (aatJ overlap)

JS43.r

tgaatccgtaatcatggtcatgaatttcttcctcgacttgttttg

aatJ-lacZ

JS35P.f

cgtctcctgtcgagtgacgaacc

aatJ-lacZ and aatQ-lacZ

JS50.f

gcatctagaccgccagtgtctgcaacc

aatQMP genes

JS51.r

gcagagctctcagtgcgggaggatcttgg

aatQMP genes

JS391K.f

aggaacttcaagatccccaattcgggaaatggtcaacgaagtgctcg

aatQM DnF-Gm

JS391K.r

tacaagaaagctgggtgatcttggcgaggaactgc

aatQM DnR-GWR

JS401K.f

tacaaaaaagcaggctggacttcagtggaatcgttcc

aatQM UpF-GWL

JS401K.r

tcagagcgcttttgaagctaattcgcgatgttcgacagcaacttgc

aatQM UpR-Gm

JS351.f

tacaaaaaagcaggctggaggagctggaggacatc

aauR UpF-GWL

JS351.r

tcagagcgcttttgaagctaattcggatgatccgttgcgccagg

aauR UpR-Gm

JS352.f

aggaacttcaagatccccaattcggaacacttcctccagcagtc

aauR DnF-Gm

JS352.r

tacaagaaagctgggtgcaggccgtacttcttcac

aauR DnR-GWR

JS100.f

tacaaaaaagcaggctccggatttcataagctggc

aatJ UpF-GWL

JS100.r

tcagagcgcttttgaagctaattcgggtaggagaagggaatcgaagc

aatJ UpR-Gm

JS101.f

aggaacttcaagatccccaattcggcgaggtcaacaagatctacg

aatJ DnF-Gm

JS101.r

tacaagaaagctgggtcctcggttcaatcggttgc

aatJ DnR-GWR

JS111.f

gcactcgaggccggggaaaaccgcgaaacaccc

aatJ gene

JS111.r

gcatctagattacatctgctcggcggccttgtcgg

aatJ gene

JS117.f

gcaggatccccggggaaaaccgcgaaacaccctgtccc

aatJQMP genes

JS117.r

gcatctagatcagtgcgggaggatcttggcgaggaactgc

aatJQMP genes

JS118.f

gcatctagagcatgtcttgctcggctgccagcaggc

aauR gene

JS118.r

gcagagctccccggcctattgcaggccgtacttcttcaccttgtcg

aauR gene

  1. aNote that oligonucleotides used in the cloning of gene-deletion plasmids were designated as DnF-Gm, DnR-GWR, UpF-GWL or UpR-Gm in accordance with the original methods publication [43]