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Table 2 Plasmids used in the current study

From: Utilization of L-glutamate as a preferred or sole nutrient in Pseudomonas aeruginosa PAO1 depends on genes encoding for the enhancer-binding protein AauR, the sigma factor RpoN and the transporter complex AatJQMP

Plasmids Relevant characteristicsa Source
pCR-Blunt Cloning plasmid; Kmr Invitrogen
pBBR1MCS-5 Broad-host-range plasmid; Gmr [42]
pDONR221 Gateway cloning plasmid; Kmr Invitrogen
pEX18ApGW Gene-deletion plasmid for P. aeruginosa; Cbr [43]
∆Plac-pBBR1MCS-5 pBBR1MCS-5 minus lac promoter; Gmr [13]
pJMS02 aauR in pCR-Blunt; Kmr This study
pJMS03 aauR in pBBR1MCS-5; Gmr This study
pJMS04 aatQMP in pCR-Blunt; Kmr This study
pJMS05 aatQMP in pBBR1MCS-5; Gmr This study
pJMS07 aatJ-lacZ in pCR-Blunt; Kmr This study
pJMS08 aatQM-lacZ in pCR-Blunt; Kmr This study
pJMS09 aatJ-lacZ in ∆Plac-pBBR1MCS-5; Gmr This study
pJMS10 aatQM-lacZ in ∆Plac-pBBR1MCS-5; Gmr This study
pJMS18 aatJQMP in pCR-Blunt; Kmr This study
pJMS19 aatJQMP in pBBR1MCS-5; Gmr This study
pJMS20 aatJ in pCR-Blunt; Kmr This study
pJMS21 aatJ in pBBR1MCS-5; Gmr This study
pJMS298 aauR::FRT-aacC1-FRT in pDONR221; Kmr Gmr This study
pJMS299 aauR::FRT-aacC1-FRT in pEX18ApGW; Cbr Gmr This study
pJMS300 aatJ::FRT-aacC1-FRT in pDONR221; Kmr Gmr This study
pJMS301 aatJ::FRT-aacC1-FRT in pEX18ApGW; Cbr Gmr This study
pJMS302 aatQM::FRT-aacC1-FRT in pDONR221; Kmr Gmr This study
pJMS303 aatQM::FRT-aacC1-FRT in pEX18ApGW; Cbr Gmr This study
  1. aNote that Cbr, Gmr and Kmr denote resistance to carbenicillin, gentamicin and kanamycin, respectively