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Table 2 Plasmids used in the current study

From: Utilization of L-glutamate as a preferred or sole nutrient in Pseudomonas aeruginosa PAO1 depends on genes encoding for the enhancer-binding protein AauR, the sigma factor RpoN and the transporter complex AatJQMP

Plasmids

Relevant characteristicsa

Source

pCR-Blunt

Cloning plasmid; Kmr

Invitrogen

pBBR1MCS-5

Broad-host-range plasmid; Gmr

[42]

pDONR221

Gateway cloning plasmid; Kmr

Invitrogen

pEX18ApGW

Gene-deletion plasmid for P. aeruginosa; Cbr

[43]

∆Plac-pBBR1MCS-5

pBBR1MCS-5 minus lac promoter; Gmr

[13]

pJMS02

aauR in pCR-Blunt; Kmr

This study

pJMS03

aauR in pBBR1MCS-5; Gmr

This study

pJMS04

aatQMP in pCR-Blunt; Kmr

This study

pJMS05

aatQMP in pBBR1MCS-5; Gmr

This study

pJMS07

aatJ-lacZ in pCR-Blunt; Kmr

This study

pJMS08

aatQM-lacZ in pCR-Blunt; Kmr

This study

pJMS09

aatJ-lacZ in ∆Plac-pBBR1MCS-5; Gmr

This study

pJMS10

aatQM-lacZ in ∆Plac-pBBR1MCS-5; Gmr

This study

pJMS18

aatJQMP in pCR-Blunt; Kmr

This study

pJMS19

aatJQMP in pBBR1MCS-5; Gmr

This study

pJMS20

aatJ in pCR-Blunt; Kmr

This study

pJMS21

aatJ in pBBR1MCS-5; Gmr

This study

pJMS298

aauR::FRT-aacC1-FRT in pDONR221; Kmr Gmr

This study

pJMS299

aauR::FRT-aacC1-FRT in pEX18ApGW; Cbr Gmr

This study

pJMS300

aatJ::FRT-aacC1-FRT in pDONR221; Kmr Gmr

This study

pJMS301

aatJ::FRT-aacC1-FRT in pEX18ApGW; Cbr Gmr

This study

pJMS302

aatQM::FRT-aacC1-FRT in pDONR221; Kmr Gmr

This study

pJMS303

aatQM::FRT-aacC1-FRT in pEX18ApGW; Cbr Gmr

This study

  1. aNote that Cbr, Gmr and Kmr denote resistance to carbenicillin, gentamicin and kanamycin, respectively