Table 2 Oligonucleotides used in the study
From: FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant
Primer name | Sequence (5′-3′)a | Purpose |
|---|---|---|
AcfR-UF-KpnI | GGGGTACCCTGATGACCCTCAGCCAGTTG | ΔacfR mutant construction |
AcfR-UR-NdeI | TCCATATGTGGATCGTTTGACGAGGTCAC | ΔacfR mutant construction |
AcfR-DF-ApaI | TCGGGCCCGAGACGCGTCGGAATTCCTGA | ΔacfR mutant construction |
AcfR-DR-AgeI | CGACGCGTAATGGCGATGCCAACTACCGA | ΔacfR mutant construction |
AcfR-CF-KpnI | GGGTACCTGGGTGCGACGCTA TCACGGA | ΔacfR-C construction |
AcfR-CR-BamHI | CGGGATCCCTGACGGCAGACATCGAAACG | ΔacfR-C construction |
AcfR-F | GTGACCTCGTCAAACGATCCA | Validation of acfR |
AcfR-R | TCAGGAATTCCGACGCGTCTC | Validation of acfR |