Table 2 Oligo nucleotides used in this study

BamHI-xreR2-FA fwd

GCCGGGATCCGTACTTATCAGTTACCACCAACCC

xreR2-FA-ovl-FB rev

CGTCAGTAGATCCATTAATTAATCCTCCGTGTTAC

xreR2-FB-ovl-FA fwd

GATCTACTGACGTAGGTGGCTGTAAATTAAAGTGG

xreR2-FB-EagI rev

ATCCCGGCCGGCGCTTCAACTAAAGGAATAGCC

EcoRI-xreR1-FA fwd

GCCGGAATTCGGTTATCTGAACGATCCTGAAC

xreR1-FA-ovl-FB rev

ATCTGTATCGCGCATTCGATAAGTATCGAACGATG

xreR1-FB-ovl-FA fwd

CGCGATACAGATTAGTGATCTATACCTTATGG

xreR1-FB-EagI rev

ATCCCGGCCG GAAATTGCGCTCGTTACTGCTG

PstI-lacI_Ptac fwd

GCGCTGCAGCATTAATTGCGTTGCGCTCA

Ptac-ovl-blank rev

ATCTGTATCGCGAGAATTCCCTCCTGTGTGAAATTG

xreR2-ovl-Ptac fwd

TCGCGATACAGATATGAAAAATCAAGACTGGCACC

xreR2-EagI rev

ATCCCGGCCGCTACTTTGTGTACCTCGAAGGCC

xreR1-ovl-Ptac fwd

TCGCGATACAGATATGAAAAAACCTAACACGATAAAATC

xreR1-EagI rev

ATCCCGGCCGCTAACTCTTTTTCTCCGCGTC

BamHI-FA 4295 fwd

GCCGGGATCCCTTATCAGACCACGGACTATGAG

FA ovl FB 4295 rev

CGTCAGTAGATCCATAGCACACCTCAGAAACAC

FB ovl FA 4295 fwd

GATCTACTGACGTAAATGCAATTTGTTGTTTATC

FB 4295-EagI rev

ATCCCGGCCGCAACGCTGGGCGCAGGCAAACCTAC).

BamHI-FA 4296 fwd

GCCGGGATCCGCATCATCGACCCTTGCCAATAC

FA ovl FB 4296_rev

CGTCAGTAGATCTTACCAGTTCCTGTTTTCATC

FB ovl FA 4296 fwd

GATCTACTGACGTCAGTTTTCTTCATTTGTGCCGCTCTG

ccdB-like ovl Ptac fwd

TCGCGATACAGATATGCAATTTGTTGTTTATC

ccdB-like-EagI rev

ATCCCGGCCGTTACAGAAAAATATTCATACAG

NheI-ccdB-like fwd

GCTGCTAGCATGCAATTTGTTGTTTATC

ccdB-like-XmaI rev

CAGCCCGGGTTACAGAAAAATATTCATACAG

check-pNPTS fwd

TGCTTCCGGCTCGTATG

check-pNPTS rev

GTAAAACGACGGCCAGTCC

check-pPINT fwd

CCGTTCTGTGCGAATCGTGGAG

check-pPINT rev

GGCCATTGGCACTGATTG

check-pBAD24 fwd

GCCGTCACTGCGTCTTTTACTGG

check-pBAD24 rev

CGCTACGGCGTTTCACTTCTG

Cy5-PxreR2 fwd

[Cy5] CTGATAAGTATCGAATGATGTTAC

PxreR2 rev

GGGTGCCAGTCTTGATTTTTC

Cy5-PxreR1 fwd

[Cy5] CTTAATAAATAATACTTGCGACCAGATTGG

PxreR1 rev

CGTGTTAGGTTTTTTCATTCGATAAG

Cy5-PpTAS fwd

[Cy5] GTGGCTGTAAATTAAAGTGGAGTAG

PpTAS rev

GTTTCATAGCACACCTCAGAAAC

Cy5-PantA fwd

[Cy5] TAATGCAGAAATTATTGCT

PantA rev

CTGAACTATTCCTATCGTTA

Cy5-PpluxC fwd

[Cy5] TTTGTATATAAAGAAGAGCTTG

PluxC

ATTAGCCATCCATTTAATG

Cy5-fryB-300 fwd

[Cy5] GGTGTATCTCATCGTCCATGACAAT

fryB −0 rev

TTGAATTCCGTTAATTCCTCGTTCAG

Btn-PxreR2 fwd

[Btn] CTGATAAGTATCGAATGATGTTAC

Btn-PxreR1 fwd

[Btn] CTTAATAAATAATACTTGCGACCAGATTGG

Btn-PpTAS fwd

[Btn] GTGGCTGTAAATTAAAGTGGAGTAG

BamHI_xreR1 fwd

GCTGGATCCGATGCAATTTGTTGTTTATC

BamHI_xreR2 fwd

CTGGATCCATGAAAAATCAAGACTGGCACCC

xreR1_XmaI rev

CAGCCCGGGTTACAGAAAAATATTCATACAG

xreR2_XmaI rev

CAGCCCGGGCTACTTTGTGTACCTCGAAGGC