Fig. 7
Effect of the pTAS components CcdA-like CcdB-like on the growth of P. luminescens 1° and 2° cells. To analyze whether CcdB-like also acts as a toxin was overexpressed ccdB-like in P. luminescens 1° as well as 2° cells. Furthermore, we deleted the cognate putative anit-toxin encoding gene ccdA-like in both cell forms and measured growth. Putative effects on the bacterias’ fitness were analyzed by measuring growth over time comparing 1° wildtype (1° wt) to the toxin overexpressing strain (1° + PtacccdB-like) and the strain lacking the antitoxin 1° ΔccdA-like (a) as well as 2° wildtype (2° wt) to the toxin overexpressing strain (2° + PtacccdB-like) and the strain lacking the antitoxin 2° ΔccdA-like (b). Additionally, ccdB-like was overexpressed in E. coli cells and growth was monitored growth on agar plates (c)