Fig. 3From: HilE is required for synergistic activation of SPI-1 gene expression in Salmonella enterica serovar TyphimuriumFormate and yeast extract increase hilA expression in both the wild type (a) and ΔhilE mutant (b); propionate increases hilA expression in both the wild type (a) and ΔhilE mutant but the further addition of yeast extract (YE) reduces it. Expression was determined by measuring β-galactosidase activity using transcriptional fusions of the hilA promoter to LacZ during growth in TB medium with the specified amount of yeast extact added. Sodium formate and sodium propionate were added at concentrations of 10 mM. Results show the mean and standard deviation from three biological replicatesBack to article page