Headspace analyses using multi-capillary column-ion mobility spectrometry allow rapid pathogen differentiation in hospital-acquired pneumonia relevant bacteria

Table 2 VOC signals that changed statistically significant in at least one bacterial species of the HAP panel

VOC No.	Substance name (or position)	1/K₀ [Vs/cm²]	RT [s]
Positive ion mode
1	Ethanol	0.509	3.7
2	P_745_4	0.745	4.3
3	P_810_5	0.81	4.7
4	P_612_6	0.612	6.3
5	P_678_7	0.678	6.7
6	P_720_16	0.72	16
7	P_508_17	0.508	16.7
8	P_685_20	0.685	19.9
9	P_603_25	0.603	25
10	P_669_25	0.669	25.2
11	P_726_25	0.726	25.2
12	P_756_25	0.756	25.2
13	2-Phenylacetaldehyd	0.616	30.4
14	P_648_36	0.648	36
15	P_580_42	0.58	41.8
16	Octan-1-ol (monomer)	0.722	44.3
17	Octan-1-ol (dimer)	0.929	44.3
18	Nonanal	0.732	53.5
19	P_748_54	0.748	53.5
20	P_634_57	0.634	56.6
21	P_755_106	0.755	106
22	P_763_127	0.763	127
23	Decan-1-ol	0.788	256.6
Negative ion mode
24	P_528_6	0.528	6.3
25	P_632_7	0.632	6.8
26	P_631_10	0.631	10.3
27	P_587_28	0.587	28.4
28	P_613_29	0.613	29
29	P_621_42	0.621	42.3
30	Indole	0.609	239

Unknown substances were named according to their position in the 2D-topographic plot as “P_x_y”, whereas “x” is representing the inverse ion mobility × 1000 and “y” is representing the MCC retention time (e.g. P_685_20 = 0.685 Vs/cm²; 20 s).
1/K₀ Drift time in Ion mobility spectrometry [Vs/cm²], RT Retention time [s]

ISSN: 1471-2180