Identification of promoter elements in the Dolichospermum circinale AWQC131C saxitoxin gene cluster and the experimental analysis of their use for heterologous expression

Table 1 Characteristics of promoter regions in the sxt biosynthetic gene cluster of D. circinale AWQC131C

Promoter	Promoter sequence 5′ ➔ 3′	Position^Δ
PsxtD	tgtcTTGTGG....(14 bp).....GAgTATACTtgactagtA	−32
PsxtP	gtatCTATCA....(12 bp).....GTgTATACTagtcaagtA	−34
PsxtPER1	ttccTTGCAA....(15 bp).....AGtTACAATtacatgA	−91
PsxtPER2	tgagATGACA....(21 bp).....CGaTATATTttgggtG	+ 94
Porf24	aaaaTTTCCT....(15 bp).....TGcTATAATgaaatcT	−160
E. coli σ⁷⁰ consensus	....TTGACA....(14 bp).....TGnTATAAT......N

The −10 and − 35 hexamers are capitalised and conserved nucleotides are in bold print. N indicates transcriptional start site (TSS). ^Δ Position of promoter relative to TSS

ISSN: 1471-2180