Skip to main content
Fig. 2 | BMC Microbiology

Fig. 2

From: The Oxidoreductase DsbA1 negatively influences 2,4-diacetylphloroglucinol biosynthesis by interfering the function of Gcd in Pseudomonas fluorescens 2P24

Fig. 2

DsbA1 regulated 2,4-DAPG production in a Gac/Rsm-independent manner. a HPLC analysis of 2,4-DAPG production by the gacA mutant and the gacA dsbA1 double mutant in KB medium with 2% glucose. b The expression of rsmZ-lacZ, rsmY-lacZ, and rsmX-lacZ transcriptional fusion was determined in P. fluorescens 2P24 and its dsbA1 mutant, respectively. The experiments were performed in triplicate, and the mean values ± SD are indicated. * indicates P < 0.05. Western blot analysis was performed to detect RsmA-FLAG (c) and RsmE-FLAG (d). Three independent experiments were performed and a representative blot was shown

Back to article page