Fig. 1
From: Identification of E2 with improved secretion and immunogenicity against CSFV in piglets
Identification of signal peptides for E2 expression in baculovirus. a Amino acid sequences of different truncated E2 signal peptides. b Schematic diagrams of different transfer plasmids for recombinant baculoviruses Ac-E2 and Ac-SP-E2. c E2 expression and secretion with different signal peptides detected in Western blotting. Culture supernatant (S) from cell culture and pellet (P) of High Five cells resuspended in PBS were examined with anti-E2 mAb 6D10, and with anti-Gp64 mAb as control. All samples were loaded in equal volume. d E2 secretion levels with different signal peptides were quantified with densitometric analysis using Gel-Pro analyzer software. The results of integral optical density (IOD) stand for protein expression levels. Experiments were performed in triplicate and data are shown as mean ± SD. Statistical significance is indicated as **(P < 0.01); ***(P < 0.001); ****(P < 0.0001)