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Table 2 Gene annotation information, RNA sequencing and qRT-PCR analysis of insert fragment of B2

From: An operon consisting of a P-type ATPase gene and a transcriptional regulator gene responsible for cadmium resistances in Bacillus vietamensis 151–6 and Bacillus marisflavi 151–25

Gene

Location

NR annotation

RNA sequence Fold Change (Cd/CK)

qRT-PCR

Fold change (Cd/CK)

Start

End

orf4086

3,723,301

3,723,687

four-helix bundle copper-binding protein

−1.95

1.59

orf4087

3,723,960

3,726,575

ATPase

47.79 b

87.26 b

orf4088

3,726,588

3,726,953

transcriptional regulator

105.62 a

102.58 a

orf4090

3,727,300

3,727,605

hypothetical protein

2.06

N

orf4091

3,727,630

3,728,040

hypothetical protein

/

0.96

orf4092

3,728,051

3,728,596

cysteine ABC transporter ATP-binding protein

/

0.89

orf4093

3,728,764

3,730,818

cadmium transporter

1.85

0.89

orf4094

3,730,891

3,731,259

transcriptional regulator

/

0.89

orf4095

3,731,357

3,731,866

hypothetical protein

/

0.89

orf4096

3,732,138

3,732,377

ferrous iron transport protein A

/

0.89

orf4097

3,732,378

3,733,106

small GTP-binding protein domain-containing protein

/

0.89

orf4098

3,733,084

3,734,481

ferrous iron transport protein B

/

0.89

orf4099

3,734,546

3,734,869

small multidrug resistance family-3 protein

/

0.89

orf4100

3,734,912

3,736,732

MULTISPECIES: hypothetical protein

/

0.89

orf4101

3,736,938

3,737,783

hypothetical protein

−2.71

N

orf4102

3,737,798

3,738,649

cation diffusion facilitator family transporter

/

2.29

orf4104

3,738,917

3,739,834

YIEGIA protein

/

14.19

orf4105

3,739,859

3,740,047

hypothetical protein

/

N

orf4106

3,740,119

3,740,538

disulfide bond formation protein B

13.10 b

12.68 b

orf4107

3,740,539

3,741,201

Protein-disulfide isomerase

12.17 b

9.07

orf4108

3,741,422

3,741,952

thiol-disulfide oxidoreductase

372.96 a

156.64 a

orf4109

3,742,073

3,742,675

cytochrome C biogenesis protein CcdA

194.33 a

130.84 a

orf4111

3,743,680

3,745,806

copper-translocating P-type ATPase

/

1.20

orf4112

3,745,799

3,746,164

cadmium efflux system accessory protein CadC

/

0.59

orf4113

3,746,292

3,746,906

cadmium resistance protein CadD

/

0.89

orf4116

3,747,815

3,748,807

oxidoreductase

/

0.73

orf4117

3,749,310

3,750,713

hypothetical protein

/

0.77

orf4118

3,750,703

3,750,882

hypothetical protein

/

N

orf4119

3,750,879

3,752,096

alpha-hydroxy-acid oxidizing enzyme

/

0.57

orf4120

3,752,206

3,752,757

hypothetical protein

−2.12

0.87

orf4121

3,752,778

3,753,803

ion transporter

/

0.69

  1. /represent the fold change of transcriptome is not significant; N represent qRT-PCR is not performed because the target genes are too small; arepresent the fold change of target gene > 100; b represent the fold change of target gene > 10