The role of Zur-regulated lipoprotein A in bacterial morphology, antimicrobial susceptibility, and production of outer membrane vesicles in Acinetobacter baumannii

Table 1 DD-CPase activity of the recombinant ZrlA protein

Substrate	Enzymatic activity of rZrlA protein (U/mg)^a
D-Ala-D-Ala	9.6 ± 4.4
Acetyl-L-Lys-D-Ala-D-Ala	6.5 ± 4.2
N_α,N_ε-diacetyl-L-Lys-D-Ala-D-Ala	34.9 ± 3.8

^aEnzymatic activity (U/mg protein) of recombinant ZrlA proteins was determined using the fluorimetric o-phthaldialdehyde method. Recombinant ZrlA protein (40 μg) was added to 10 mM solutions of indicated substrates. The results are the mean ± SD. Assays were performed in triplicate

ISSN: 1471-2180