Microbiology of secondary infections in Buruli ulcer lesions; implications for therapeutic interventions

BMC Microbiology

Table 1 List of Primers used for PCR Amplification

Primers	Forward and reverse sequences (5’-3’)	Expected sizes (bp)	References
1_16SF	F-AGGAGGTGATCCAACCGCA R-AACTGGAGGAAGGTGGGAT	350	[22]
16SrRNA	MSHA-AAAAAGCGACAAACCTACGAG PA-AGAGTTTGATCCTGGCTCAG	620	[23]
IS2404 (nested 1)	pGp1: AGGGCAGCGCGGTGATACGG pGp2: CAGTGGATTGGTGCCGATCGAG	400	[24, 25]
IS2404 (nested 2)	pGp3: GGCGCAGATCAACTCGCGGT pGp4: CTGCGTGGTGCTTTACGCGC	200	[24, 25]
ITS	ITS1-TCCGTAGGTGAACCTGCGG ITS4-TCCTCCGCTTATTGATATGC	variable	[26]

ISSN: 1471-2180