Fig. 1From: Differential production and secretion of potentially toxigenic extracellular proteins from hypervirulent Aeromonas hydrophila under biofilm and planktonic cultureGeneral proteolytic potential of vAh extracellular proteins (ECPs) secreted under biofilm and planktonic growth. The general proteolytic potential of biofilm and planktonic secretomes was measure using HiLyteFluor 488-labeled casein as a substrate. Secreted protein from each condition was incubated at 30 °C with labeled casein and fluorescent intensity was measured at Ex/Em = 490 nm/520 nm every five minutes for one hour. Data were plotted as relative fluorescence units versus time for each sample. Trypsin served as a positive control, and sterile, deionized water served as a negative control. Three individual experiments were performed, and all samples were performed in triplicate. Statistical analysis consisted of one-way ANOVA followed by Tukey’s multiple comparisons post-test with significance set at p < 0.05Back to article page