Table 2 Primers used for hpa1 deletion mutant constructions
Primer names | Primer sequence (5′-3′) | Purpose |
|---|---|---|
HpaXmΔL39A-F | CATCTCGGAAAAGCAGGCCGACCAGCTGCTGACCC | Primers used to construct hpaXmΔL39A |
HpaXmΔL39A-R | GGGTCAGCAGCTGGTCGGCCTGCTTTTCCGAGATG | |
Hpa1XooΔD41A-F | CTCGGAAAAGCAACTGGCTCAGTTGCTGTGCCAGC | Primers for constructing hpa1XooΔD41A |
Hpa1XooΔD41A-R | GCTGGCACAGCAACTGAGCCAGTTGCTTTTCCGAG | |
HpaXpmΔM54A-F | ACACAGCTCATCATCCTGGCCCTGCTGC | Primers for constructing hpaXamΔM54A |
HpaXpmΔM54A-R | GGATGATGAGCTGTGTCAGCAACTGGTC | |
HpaXcmΔL40A-F | CATCTCGGAAAAGCAAGCCGACCAGCTGCTGACCC | Primers for constructing hpaXcmΔL40A |
HpaXcmΔL40A-R | GGGTCAGCAGCTGGTCGGCTTGCTTTTCCGAGATG |