Fig. 1

Ultrastructure of yeast cells before and after 14-day desiccation. a Wild type cells growing in YPD medium for overnight to log-phase, then processed for TEM observation without drying. b Wild type cells growing in YPD medium for 3 days to stationary phase, then processed for TEM without drying, showing abundance of mitochondria, the nucleus-vacuole junctions (arrows) and multiple vacuoles (B1), and single vacuole (B2). c Wild type cells growing in YPD medium for overnight to log-phase, then dried for 14 days, showing circular ER membrane (arrows), and increased gap between inner and outer nuclear membrane. d Wild type cells growing in YPD medium for overnight to log-phase, then dried for 14 days, showing a dividing mother (left) and daughter cell (right). ER membrane forms circular structure (arrow) but mostly observed in the mother cell. N = nucleus, V = vacuole, LD = lipid droplet; m = mitochondrion. Inset shows the enlarged image of the boxed area in the same image