Fig. 2
From: Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis
Fe-S cluster reconstitution of purified IscU2. a SDS-PAGE analysis of purified IscU2 (cropped image of original). Lane 1, MW marker; lane 2, IscU2 (2.4 μg). b Anaerobic UV-visible spectra of 20 μM IscU2 (dotted line), IscU2S-FeS (black line) and IscU2C-FeS (gray line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Anaerobic size exclusion chromatography of 14.8 mg of IscU2 (gray line) and 15.6 mg of IscU2C-FeS (black line) in 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscU2 and IscU2C-FeS were calculated with a standard curve (inset). The calculated MW of IscU2 (solid square symbol) was 12 kDa and the calculated MW of IscU2C-FeS (open square symbol) was 26 kDa