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Fig. 6 | BMC Microbiology

Fig. 6

From: The combined antibacterial effects of sodium new houttuyfonate and berberine chloride against growing and persistent methicillin-resistant and vancomycin-intermediate Staphylococcus aureus

Fig. 6

SNH led to a decreased potential and an increased permeability of cell membrane in S. aureus ATCC33591. Bacterial membrane potential (a) was represented by the ratio of red/green fluorescence of DiOC2(3). CCCP (5 μM) was used as the positive control in the DiOC2(3)-based membrane potential assay. Bacterial membrane permeability (b) was measured by TO-PRO-3. Nisin (25 μg/mL, green) was used as a positive control in the TO-PRO-3-based membrane permeability assay. Untreated ATCC33591 cells were regarded as a negative control (black) in both assays. Cells treated with 1/4 MIC SNH, 1/8 MIC BBR and the SNH-BBR combination were presented as blue, orange and red, respectively. Asterisks denote statistical significance as determined by one-way ANOVA followed by Tukey’s multiple-comparison analysis ****, P < 0.0001

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BMC Microbiology

ISSN: 1471-2180

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