Fig. 2
From: The combined usage of Matrine and Osthole inhibited endoplasmic reticulum apoptosis induced by PCV2
Replication of PCV2 in PK-15 cells. PK-15 cells were infected with 104.4 TCID50 of PCV2. Virus copy numbers were detected by qRT-PCR from the samples collected at 6, 12, 24, 48, 72 and 96 h after the initial infection. The chart plotted with virus copy number against the time points after the infection was generated, which showed that the virus replication reached a plateau at 48 h after infection. The virus number was very low in the first 24 h of infection and then dramatically increased between 24 h and 48 h after infection, reaching a plateau at 48 h. Therefore, all samples were collected at 48 h after infection to analyze the anti-viral effect and anti-apoptotic mechanisms of the compounds