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Fig. 6 | BMC Microbiology

Fig. 6

From: Short chain fatty acids induced the type 1 and type 2 fimbrillin-dependent and fimbrillin-independent initial attachment and colonization of Actinomyces oris monoculture but not coculture with streptococci

Fig. 6

Effects of a mixture of acetic acid, propionic acid and butyric acid on INAC. INAC areas of wild-type A. oris MG1 and MG1 ΔfimA by live cells and with dead cells were observed in the control, with no acid; with 60 mM butyric acid treatment; mixture of 5 mM butyric acid, 15 mM propionic acid and 40 mM acetic acid treatment; and under the pH 4.7 condition prepared with HCl in mixture of 5 mM butyric acid and 15 mM propionic acid, and 20 mM butyric acid. The attached and colonized cells were stained using a LIVE/DEAD staining kit and were observed by CLSM at the edges of the wells. All CLSM images were obtained with a 10× objective. Images were further analysed to determine the live and dead cell areas in MG1 using ImageJ. Representative data from more than three independent experiments are presented in each panel

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BMC Microbiology

ISSN: 1471-2180

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