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Table 2 Characteristics of the XAC1051-2qPCR assay performed using three different devices

From: Development and comparative validation of genomic-driven PCR-based assays to detect Xanthomonas citri pv. citri in citrus plants

  Plant matrix StepOnePlus QS LC480
qPCR Efficiency lemon 93% 94% 97%
orange 102% 103% 106%
R2 lemon 0.992 0.981 0.984
orange 0.988 0.986 0.987
Intra-assay variation Cv range (median)   0.07–2.48%
(0.34)
0.13–4.6%
(0.60)
0.14–3.6%
(0.81)
Inter-assay variation Cv range (median)   0.49–2.5%
(1.50)
0.55–3%
(1.30)
0.84–2.4%
(1.50)
Cut-off values   35.9 38.43 38.89
LOD95%
(CI)
  2.90
(2.76–3.04)
3.04
(2.96–3.11)
3.09
(2.92–3.25)
Vic Ct range   25.8–31.2 22.3–27.9 23.4–29.7