Table 2 Characteristics of the XAC1051-2qPCR assay performed using three different devices

qPCR Efficiency

lemon

93%

94%

97%

orange

102%

103%

106%

R²

lemon

0.992

0.981

0.984

orange

0.988

0.986

0.987

Intra-assay variation Cv range (median)

0.07–2.48%

(0.34)

0.13–4.6%

(0.60)

0.14–3.6%

(0.81)

Inter-assay variation Cv range (median)

0.49–2.5%

(1.50)

0.55–3%

(1.30)

0.84–2.4%

(1.50)

Cut-off values

35.9

38.43

38.89

LOD95%

(CI)

2.90

(2.76–3.04)

3.04

(2.96–3.11)

3.09

(2.92–3.25)

Vic Ct range

25.8–31.2

22.3–27.9

23.4–29.7