Fig. 1

Standard curve obtained for a dilution series of the X. citri pv. citri strain IAPAR 306 in a sweet orange matrix. XAC1051-2qPCR was run on total DNA extracted from sweet orange leaves spiked with serially suspensions obtained from 10-fold serial dilution (10⁷ to 10² CFU ml^− 1). The standard curve was constructed using linear regression analysis of the threshold cycle (Ct) values for the serial dilutions over the Log₁₀ of the initial target concentrations (compilation of all series and runs, corresponding to 9 replicates at each concentration level). The linear regression equation, the efficiency value and the adjusted R² are indicated